文献：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163

The insolubility of a PM molecule upon cold-Triton treatment has often been associated with its partitioning into raft domains in the live-cell PM, although this has never been proved (6 7, 20; reviewed in 32). Therefore, in this research, we examined the cold-Triton solubilities of Bdp-Chol and other phospholipid probes incorporated in the PM, for comparisons with those of other prototypical raft and non-raft molecules in the literature, such as GPI-anchored proteins (e.g. CD59, Thy1 and DAF) and transferrin receptor, respectively. The HASM and COS-7 PMs that incorporated Bdp-Chol, fluorescent phospholipid probes, [Cy3-conjugated phosphatidylethanolamines (Cy3-PEs)], collectively representing Cy3-dioleoyl-PE, Cy3-dimyristoyl-PE and Cy3-dipalmitoyl-PE, abbreviated as Cy3-DOPE, Cy3-DMPE and Cy3-DPPE, respectively) or Bodipy FL (Life Technologies)-conjugated GM1 in its alkyl chain (Bdpø-GM1), were extracted with cold Triton (1%, at 2.8°C for 15 min; Figure S1). Bdp-Chol, as well as the prototypical raft-associated molecular complex of the endogenous ganglioside GM1 and Cy3-conjugated cholera toxin B subunit, mostly remained in the DRMs. In contrast, all three species of Cy3-PEs and Bdpø-GM1 were almost entirely solubilized; i.e. Cy3-DMPE, Cy3-DPPE and Bdpø-GM1 are cold-Triton soluble, like the prototypical non-raft molecule Cy3-DOPE. Cy5-DMPE 36 and Bdpø-GM1 21 were previously assumed to be raft-associated without any experimental data, but the results obtained here indicate that these molecules are not prototypical DRM-associated molecules. The present results are consistent with the data obtained by Sezgin et al. 24. Meanwhile, the partitioning of Bdp-Chol in DRM suggested that Bdp-Chol could be a good analogue for native cholesterol, consistent with previous data (see Introduction).

在这项研究中，我们检查了Bdp-Chol和PM中掺入的其他磷脂探针的冷Triton溶解度，分别与文献中其他原型筏和非筏分子的溶解度进行比较，如GPI锚定蛋白（如CD59、Thy1和DAF）和转铁蛋白受体。

将含有Bdp-Chol、荧光磷脂探针[Cy3-共轭磷脂酰乙醇胺（Cy3-PE）]的HASM和COS-7 PM（统称为Cy3-dioleoyl-PE、Cy3-dimyristoy-PE和Cy3-dipalmitoy-PE，分别缩写为Cy3-DOPE、Cy3-DMPE和Cy3 DPPE）或其烷基链中的Bodipy FL（Life Technologies）共轭GM1（Bdpø-GM1）用冷Triton（1%，2.8°C）提取15分钟 min）。

Bdp-Chol以及内源性神经节苷脂GM1和Cy3结合的霍乱毒素B亚基的原型筏相关分子复合物大多留在DRMs中。相比之下，所有三种Cy3-PE和Bdpø-GM1几乎完全溶解；即Cy3-DMPE、Cy3-DPPE和Bdpø-GM1是冷溶于Triton的，就像典型的非筏分子Cy3-DOPE一样。

Cy5-DMPE 36和Bdpø-GM1 21之前被认为与筏相关，但没有任何实验数据，但这里获得的结果表明，这些分子不是典型的DRM相关分子。目前的结果与Sezgin等人24获得的数据一致。

相关推荐：

DSPE-PEG-cisplatin

DSPE-PEG-CLS

DSPE-PEG-COOH CAS No.: 1403744-37-5

DSPE-PEG-Cyanur

DSPE-PEG-DA

DSPE-PEG-Dopamine

DSPE-PEG-DOTA

DSPE-PEG-Estrogen

DSPE-PEG-Galactose

以上文章内容来源各类期刊或文献，如有侵权请联系我们删除！