Cy3-DOPE在完整细胞质膜中的运动动力学研究-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨推荐西安齐岳生物 -

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Cy3-DOPE在完整细胞质膜中的运动动力学研究

发布时间：2025-07-04 作者：zyl 分享到：

文献：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163

Using the PtK2 cell line, which was previously employed by Eggeling et al. 22 and Sahl et al. 23, we examined the behaviors of Bdp-Chol and Cy3-DOPE in the top PM. Their typical trajectories are shown in Figure 1. A clearly remarkable feature of the trajectories in the intact cells, shown in Figure 1A, is that the tracking of a single Bdp-Chol molecule in the intact PM required an enhanced time resolution, from 33 milliseconds to 0.5 milliseconds, and that to place the typical trajectories of both Bdp-Chol and Cy3-DOPE in similar sizes, the trajectory lengths had to be different; i.e. 50- and 500-millisecond observation durations (at 0.5- and 33-millisecond resolutions for 100 and 15 steps, respectively). This clearly shows that Bdp-Chol diffused much faster than Cy3-DOPE.

Cy3-DOPE

我们研究了Bdp-Chol和Cy3-DOPE在顶部PM中的行为。

完整细胞中轨迹的一个明显显著特征是，在完整PM中追踪单个Bdp-Chol分子需要提高时间分辨率，从33 毫秒到0.5 毫秒，为了使Bdp-Chol和Cy3-DOPE的轨迹大小相似，轨迹长度必须不同；即50毫秒和500毫秒的观测持续时间（分别以0.5毫秒和33毫秒的分辨率进行100步和15步）。这清楚地表明，Bdp-Chol的扩散速度比Cy3-DOPE快得多。